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Description

The liver serves as a primary role to the understanding of pharmacokinetics, driving the need for effective and reproducible liver models. While animal liver models and HepG2 cell lines offer accessibility and replicability, they lack functional accuracy in comparison to human livers. Primary human hepatocytes, often considered the “golden standard” for in vitro modeling due to high metabolic function and capabilities, face challenges in scalability and long-term function. These limitations hinder the scalability of successful primary hepatocyte models, impairing their use as a rapid response tool to chemical threats. Immortalized hepatocytes are liver cells that have been genetically modified to indefinitely divide while still maintaining normal liver cell characteristics and function. Cell modeling with immortalized hepatocytes has been relatively unexplored but shows promising potential to bridge the gap between reproducibility and quality cell function. Our study aims to provide an effective liver organoid model within a microfluidic system to build upon the underlying potential of these hepatocytes. Preliminary findings serve as characterization of the hepatocytes to be used in our model and further exploration of their capabilities for essential cell function.

Publication Date

4-1-2025

Keywords

immortalized hepatocytes, liver organoid model, microfluidic system, drug

Advancing Liver Models: Exploring Immortalized Hepatocytes for Improved Pharmacokinetic Studies

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