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Description

Salmonella is listed by the Centers for Disease Control and Prevention (CDC) as one of the most common foodborne pathogens and it is a bacterium to trigger symptomatic infections with experiencing diarrhea, fever, and stomach cramps. Genomic DNA from Salmonella as a target analyte can be used for assay development, verification, and validation in monitoring Salmonella contamination to secure food and food supply chain safety. The technology of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is a gene editing tool acting as precise molecular scissors to cut a target sequence. He et al. developed an “Amplification-by-Polymerization (AbP)” in biosensing technique based on effective mass growth upon biomolecular recognition using reversible-deactivation radical polymerization reactions and successfully demonstrated direct visualization of DNA, protein, and other biomarkers via AbP processes. This interdisciplinary collaborative research is aimed at developing a novel CRISPR-integrated Amplification-by-Polymerization method to detect Salmonella for human health and food safety protection in a CRISPR-Cas12a and biosensing-based, rapid, sensitive, label-free, PCR-free, and detector-free fashion.

Publication Date

4-1-2025

Keywords

Salmonella detection, foodborne pathogens, genomic DNA, CRISPR-Cas12a, biosensing, Amplification-by-Polymerization, AbP, food safety, assay development, gene editing, molecular diagnostics, PCR-free detection, label-free detection, pathogen monitoring, food supply chain, interdisciplinary research, public health, biomolecular recognition, rapid diagnostics, CRISPR technology

Development of CRISPR-Integrated Amplification-by-Polymerization for Detection of Salmonella

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