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Description

Nuclear Factor of kappa B (NF-κB) is implicated in many diseases and is a target for therapeutic interventions. Many anti-inflammatory pharmacological drugs are derived from plants. Moringa oleifera or the “miracle tree” has health benefits. Moringa extracts have been shown to inhibit inflammatory responses in macrophages through a mechanism involving NF-κB. Moringa also impacts T cell function, but whether it inhibits NF-κB activation in T cells is unknown. Our lab is interested in characterizing the impact of Moringa oleifera on T cells. To specifically investigate the impact of Moringa on NF-κB activation in T cells, we will culture Jurkat E6.1 T cells [5x10 6 cells/ml] in complete RPMI pre-treated with ethanolic Moringa extracts (MOE) [0%, 0.5%, 1%, 2.5%, and 5% w/v] for 1 hour. Cells will be stimulated with anti-CD3 and CD28 [1 mg/ml] for 15, 30, and 60 min. Nuclear and cytosolic extracts will be assayed by Western blotting to assess NF-κB activation. Data from this study will contribute to gaps in knowledge related to how Moringa impacts the function of T cells, key component of the adaptive immune responses; ultimately contributing to the growing knowledge of how Moringa oleifera serves as a promising therapeutic alternative.

Publication Date

4-1-2025

Keywords

NF-κB, Nuclear Factor kappa B, T cells, Moringa oleifera, Moringa extracts, anti-inflammatory, Jurkat E6.1 cells, adaptive immune response, plant-based therapeutics, CD3/CD28 stimulation, Western blotting, inflammation, immune modulation, natural medicine, pharmacognosy, cytokine signaling, herbal therapy, immune cell function

Investigating the Role of Moringa oleifera in NF-kB Inhibition in T Cells

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