Date of Award

2014

Document Type

Thesis

First Advisor

Worku, Mulumebet

Abstract

Gastrointestinal nematodes in goats have developed resistance to chemical anthelmintics, resulting in the need for alternative control strategies. One approach is incorporation of Sericea Lespedeza (SL) as high quality forage that suppresses these parasites in goats due to its high condensed tannin (CT) content. However, little is known about its effect on rumen microorganisms and innate immunity in goats. A diverse collection of microorganisms is found in the goat rumen and Bifidobacteria is one of the important organisms in the immunity, yet its presence in the goat rumen is not fully studied. The objective of this study was to evaluate the impact of a diet containing SL on goat rumen microorganisms, Bifidobacteria and on the goat’s innate immune gene expression. Microbial DNA was isolated from rumen samples using the QIAamp DNA kit (Qiagen, USA) to compare rumen microbes and Bifidobacteria in control group and group that received SL in diet.

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