Date of Award

Spring 2015

Document Type

Thesis

First Advisor

Fakayode, Sayo O.

Abstract

The negative health impact of chiral environmental pollutants (CEPs), including mutagenicity, teratogenicity, and developmental problems, highlight the importance of chirality to the biomedical research community and the need for new techniques for accurate analysis of CEPs in humans to promote the development of biomedical protocols for CEPs poisoning therapy. This study examined the effects of chirality on 1-(9-anthryl)-2,2,2-triflouroethanol (TFE) and 1,1′binaphthyl-2,2′-diamine (BNA) enantiomers interactions with human serum albumin (HSA), the dominant serum protein and a transporter of hormones, drugs and metabolites in human. Specifically, the emission, the binding constants, stoichiometry, and thermodynamic properties of enantiomers of TFE-HSA and BNA-HSA complexes were investigated using analytical spectroscopy (Fourier transform infrared, UV-visible and fluorescence). The influence of experimental conditions such as the temperature and the concentration of TFE and BNA enantiomers on TFE-HSA and BNA-HSA complexations were also investigated. In addition, the effect of TFE and BNA enantiomers on the mobility of HSA in solution was explored by the use of steady state fluorescence anisotropy measurement. Furthermore, enantiomeric cytotoxicity and cell viability of TFE and BNA enantiomers on triple negative breast cancer cells was investigated. The results of this study showed considerable differences in the emission property, the binding constants, and thermodynamic properties of the enantiomers TFE-HSA and BNA-HSA complexes, indicating diastereomeric complex formations and chiral discrimination. The result of the study also showed differences in the TFE and BNA enantiomeric cell cytotoxicity and cell viability on triple negative breast cancer cells.

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