Date of Award


Document Type


Degree Name

Master of Science (MS)


Chemical Engineering

First Advisor

Lou,Jianzhong Dr.


Molecular imprinting is a procedure for the preparation of artificial receptors for specific binding with target molecule. In this work molecularly imprinted polymer (MIP) film for the recognition of bilirubin was synthesized and studied using the Quartz Crystal Microbalance (QCM). The MIP was coated on the QCM crystals and analyzed using QCM. 4-vinylpyridine was used as functional monomer, divinylbenzene as cross-linking agent and benzophenone as initiator. As a control, another polymer was prepared in the same way as the MIP but without the template monomer. The control polymer or the non-MIP exhibited very little affinity for the target molecule compared to the MIP. A calibration curve for bilirubin concentration was obtained from the frequency shifts given by different concentrations of bilirubin solution using the QCM. The effects of pH and ionic strength on recognition were also discussed. To ensure that the MIP synthesized was selective, biliverdin recognition was studied and compared to that of bilirubin. The MIP showed a higher affinity for bilirubin than biliverdin.