Date of Award
Biomarkers are molecules that indicate changes in a physiological state and are detected by biosensors. Aptamer based biosensors are highly efficient, with high specificity and reusability. An aptamer library for a 25-mer aptamer contains 1015 possible sequences. Experimentally, the procedure known as Systematic Evolution of Ligands by Exponential Enrichment (SELEX) is used. Selecting an aptamer from such a huge library is highly involved and time consuming. A single round of SELEX uses a few hundred aptamers and can take few hours to weeks.
Rhinehardt, Kristen Lorraine, "Computational Modeling Of Mucin 1 (Muc1) Peptide And Anti-Muc1 Aptamer Binding" (2012). Theses. 85.