Title

The Role of Transcription Factor YY1 in the Expression of SNX3 and CPNE1

Student Classification

Junior

Faculty Mentor

Dr. Lijun Wang

Department

Biological Engineering

Document Type

Poster

Publication Date

Spring 2019

Abstract

The analysis of interactions between protein and DNA in the chromatin environment is essential to better understand the mechanisms governing gene expression in eukaryotes. Hybridization Capture of Chromatin-Associated Proteins for Proteomics (HyCCAPP) is a novel technology that identifies proteins bound to specific regions of the genome. The HyCCAPP method enables sequence-specific capture of DNA using complimentary oligonucleotides followed by identification of the proteins bound to that region using mass spectrometry. In our studies, we investigated sequence variants in the promoter regions of the human genes CPNE1 (Copine-1) and SNX3 (Sorting-Nexin-3). CPNE1 on chromosome 20 is important for calcium-dependent cell membrane association and is associated with non-small cell-lung cancer in humans. SNX3 on chromosome 6 is involved in protein trafficking. Mutation of this gene is associated with microcephaly and microphthalmia. According to the literature, transcription factor YY1 (Yin Yang 1) has an activating effect on CPNE1 and repressing effect on SNX3 in their wild type forms. The goal of our analysis was to verify the change in expression due to mutations in the YY1 binding region in the promoters of these genes. Then, using HyCCAPP we will identify YY1 and other proteins involved in regulation of the mutated forms of SNX3 and CPNE1.

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