Effect of Dietary Spray-dried Plasma on Antibiotic Susceptibility of Salmonella Enteritidis Isolates Obtained from Ceca of Challenged Broiler Chickens

Department

Department of Animal Sciences

Document Type

Poster

Publication Date

Spring 4-2021

Abstract

Salmonella spp. is the predominant bacterial cause of foodborne gastroenteritis which adapts, enters, and alters the gastrointestinal environment in humans. The increased demand by consumers for antibiotic-free poultry meat requires the continuous search for alternative growth-promoting products. Spray-dried plasma (SDP) contain growth factors, functional proteins and can stimulate the immune system with a potential to replace antibiotics in broiler diets. An experiment was conducted to determine the changes induced by dietary spray-dried plasma (SDP) on antibiotic susceptibility of Salmonella Enteritidis (SE) isolated from challenged broiler chickens. Day-old (720) Ross 708 male chicks were obtained from a commercial hatchery, weighed, and randomly assigned to 12 dietary treatments. Each treatment consisted of 4 replicate pens, with each pen housing 15 chicks. To determine the effect of dietary spray-dried plasma on antibiotic susceptibility of SE, ceca of challenged chicks were collected on d 3 and 14 of experiment. Contents were homogenized and isolated and antibiotic susceptibility was tested using four antibiotic MIC test strips (Ampicillin, Tetracycline, Bacitracin, and Erythromycin). Results showed that Ampicillin, Tetracycline, and Bacitracin inhibition of SE among the treatments was different (P < 0.05) in both d 3 and 14. However, Erythromycin showed no differences (P > 0.05) in SE inhibition in both d 3 and 14. It was concluded that dietary supplementation of SDP at 30 g/kg diet was not different from MSE treatments. Different inhibition rates were observed on the different antibiotic MIC test strips used. SDP may be able to replace antibiotics such as BMD in broiler chick diets. Keywords: Salmonella Enteritidis, Spray-Dried Plasma, Antibiotic Susceptibility, Antibiotics

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