Human Coronary Artery Endothelial Cells (HCAEC) cell’s response to C-Reactive Protein
Charles Coleman, Senior, Bioengineering Kayla E. Webster, Senior Bioengineering
Dr. Kristen Dellinger, Joint School of Nanoscience & Nanotechnology (JSNN) ; Jessica Norcott, Joint School of Nanoscience & Nanotechnology (JSNN)
Within a collaborative investigation in the Dellinger Lab, I have been assisting with the growth of lung cells. Propagating a viable stock of Human Coronary Artery Endothelial Cells (HCAEC) cells is our first goal in preliminary investigations, to help complete the latter of the experiment. While we hope to see data depicting inflammatory responses to reactive protein concentrations, I am particularly invested in the health and preservation of the cells. To monitor the health of the cells, I feed them with essential nutrients and monitor their growth with optical microscopy techniques. Our ultimate plan of study after a viable stock of HCAEC cells has been made, is to then infect the cells with a C-reactive protein; we are trying to simulate the inflammation process in cells that occurs at the early stages of coronary artery disease. Western Blot technique will be used to analyze the inflammatory response to the C- Reactive protein.
Coleman, Charles and Webster, Kayla E., "Human Coronary Artery Endothelial Cells (HCAEC) cell’s response to C-Reactive Protein" (2023). Undergraduate Research and Creative Inquiry Symposia. 327.