Effect of Dietary Fat Types on Dopamine Concentration in the Ileum of Broiler Chickens
Shaniece Thorpe, 4th-Year, Department of Animal Science
Dr. Yewande Fasina, Dr. Adedeji Adetunji, and Paul Omaliko, Department of Animal Sciences
Department of Animal Sciences
The aim of the 8-week experiment was to evaluate the effect of different dietary fat types on oxidative status and chronic gut inflammation in vertebrates, using the chicken as a model. Experimental treatments consisted of corn-soy based diet (Control, CON), into which olive oil (OLIV), fish oil (FISH), canola oil (CANO), lard (LARD), coconut oil (COCO), and flaxseed oil (FLAX) were incorporated at 3% level to make a total of 7 dietary treatments. On d 55, plasma and ileum tissue samples were collected for biological antioxidant potential (BAP) and reactive oxygen metabolites (d-ROM) tests. Ileal tissue was also evaluated for the expression level of inflammation indicator proteins, namely, tumor necrosis factor-alpha (TNF-alpha) and tyrosine hydroxylase (TRH) genes. Results showed that birds in the COCO, CANO, and FISH treatments had higher levels (P < 0.05) of d-ROM (28.0 to 37.0 Carr U) compared to OLIV and FLAX treatments (18 t0 20 Carr U). On the other hand, the BAP values were within normal range for all treatments. The expression level of TNF-alpha gene was higher (P < 0.05) for LARD and FISH (1.93 to 1.94) compared to CON, CANO and FLAX (0.26 to 1.00). The expression level of TRH gene was higher for LARD (P < 0.05; 2.16) compared to CON, CANO, FLAX, and FISH treatments (0.27 to 1.00). It was concluded that birds in COCO, CANO, FISH, and LARD had higher stress levels, with the degree of chronic gut inflammation being higher in LARD and FISH birds.
Thorpe, Shaniece, "Effect of Dietary Fat Types on Dopamine Concentration in the Ileum of Broiler Chickens" (2023). Undergraduate Research and Creative Inquiry Symposia. 305.