Effect of Exogenous Recombinant Galectins on Total Plasma Protein Concentration in Cow Blood

Student Classification

Freshman

Faculty Mentor

Mulumebet Worku, Ph.D.

Department

Department of Animal Sciences

Document Type

Poster

Publication Date

Fall 2019

Disciplines

Animal Sciences

Abstract

Galectins are soluble proteins that can be secreted from cells. Secreted Galectins interact with glycoconjugates and play important roles in regulating immunity. Changes in total plasma protein concertation serve as indictors of immune activation. The objective of this project was to determine the effects of three exogenous recombinant Galectins on the concentration of total plasma proteins in cow blood. Blood samples (1 ml each) from three cows were treated with either recombinant Galectin 3, 4, or 9 (50ug/ml 30 min 37C in 5% Co2). Controls were left untreated. Plasma was then extracted and diluted. The concentration of total plasma protein was determined using the Pierce BCA kit (Thermo Scientific Pierce, Rockford, IL). Results were analyzed using ANOVA. Treatment with all three recombinant Galectins significantly increased the concentration of total plasma protein (F=6.6, p-value=0.028). The average increases due to treatment with Gal3, Gal4, and Gal9 were 55%, 50% and 48% respectively. Exogenous galectins can modulate the levels of plasma proteins in cow blood. Further evaluations are needed to identify specific proteins and how they are regulated.

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